منابع مشابه
Cas9 Functionally Opens Chromatin
Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler transcription factor retinoic acid receptor at previously unbound motifs. Thus, we demonstrate a new use for Cas9 in increasing surrounding chromati...
متن کاملATP-dependent chromatin assembly is functionally distinct from chromatin remodeling
Chromatin assembly involves the combined action of ATP-dependent motor proteins and histone chaperones. Because motor proteins in chromatin assembly also function as chromatin remodeling factors, we investigated the relationship between ATP-driven chromatin assembly and chromatin remodeling in the generation of periodic nucleosome arrays. We found that chromatin remodeling-defective Chd1 motor ...
متن کاملCTCF induces histone variant incorporation, erases the H3K27me3 histone mark and opens chromatin
Insulators functionally separate active chromatin domains from inactive ones. The insulator factor, CTCF, has been found to bind to boundaries and to mediate insulator function. CTCF binding sites are depleted for the histone modification H3K27me3 and are enriched for the histone variant H3.3. In order to determine whether demethylation of H3K27me3 and H3.3 incorporation are a requirement for C...
متن کاملThe demise of a TUDOR under stress opens a chromatin link to 53BP1.
The mechanisms that localise 53BP1 to sites of DNA double-strand breaks (DSBs) have remained elusive, despite this protein’s key roles in DNA damage response signalling and repair processes. Recent studies, including the work by Mallette et al (2012) in this issue of The EMBO Journal, now provide crucial insights into the roles of ubiquitin-dependent signalling cascades at DNA damage sites requ...
متن کاملStructure that Opens the Gate and Opens the Door
Sometimes the best way to solve a problem is to look at it from a different perspective. That is certainly the case in ion channel research. Hodgkin and Huxley must have realized that 50 years ago when they looked at membrane currents with a voltage clamp, and Sakmann and Neher realized it 20 years ago when they measured Possible Symmetries of Tetrameric Channel single-channel currents with the...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: PLOS ONE
سال: 2016
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0152683